Help diagnose speed-related thump from rear

[Diamond_D]

Haven't driven my 4runner in a few weeks, but last time out was a long trip with 0 problems. Yesterday I took it out and when I got on the highway above 40mph or so, I started to hear a loud noise and feel a vibration.

As I slowed down, I could tell it was a repetitive thump noise that slowed down as the vehicle slowed down. Sounded like it was coming from under the rear seats or further back. It did not go away if I let off the gas, and didn't change in 2wd vs 4wd.

Video w/sound is below, seems pretty audible with volume up. I checked the driveshaft and wheels and don't see anything stuck there or obviously excessive play, etc. Would be grateful for any educated guesses on the source!

2007 4Runner Noise - YouTube

 

[r0cksal0t]

That's a tough one. The only things back there spinning are the driveshaft, rear diff, wheel bearings, and wheels. I would check lug nuts to make sure a tire isn't loose, then jack up the rear and and check wheel bearings by trying to move the tire back and forth. I don't know much about the diff or drive shaft so I'm not sure about checking those.

Since it's been a while since you posted this, have you found the issue?