i tapped the VSS on mine before it is behind the glove box. i forgot exactly which one it was i need to check again. i tapped mine because the old AVN 3543 need a VSS power source.
Thank you all for your post! @ Ripcord, running an eclipse AVN5510 navi unit. The unit freezes sometimes just running with the GPS antenna without the VSS wire hooked up. Same problem I had in my 06 Taco with the AVN6610 navi unit until i hooked up the VSS wire. Thanks again!