Yes and probably not.
What that means is, you can probably get an adapter somewhere. There were several different speaker variations on the 4th gen runner, with different Head units. I have a JBL system, which includes a factory sub, and as a result I cannot run a stock NON JBL without an adapter or splicing.
If you know anything about wiring, you can hand wire it up. If not, look around for an adapter or a universal plug. Honestly, if you can wire on your own, you can make one that will work.
Tl;DR. Yes, You can make it work. No, it will probably not just mount up. Make your own, or buy an adapter (if they make it).