I haven't occasion to lock or unlock my center diff as yet, but the manual for the 2008 V8 4WD says that center diff can be locked in both 4WD modes. Even has a logical pattern through the book like: In V8 4WD (FT4WD), First, do nothing to drive through normal roads, snowy and icy roads (try it in Normal 4WD Hi, unlocked center diff). If that doesn't work, lock the center diff while still in 4HI. If that doesn't work, try 4LO, center diff unlocked. If that doesn't work, try 4LO with center diff locked...Seriously, that's about what the manual says to do. lol
I've been studying up in "the book" on this 4Runner 4WD system since coming over from a multi-mode GM that was otherwise "shift on the fly" 4WD with the exception of shifting to 4LO. The rear diff in that had an Eaton electronic locker, and the front hubs stayed locked (but free-wheeling) in even in 2WD mode (made it hard to turn, another reason I got rid of it for the 4Runner) and when you activated 4WD it just engaged the front diff.