2010 Limited (why else would I be posting in the 5th Gen. forum?). Bought new, then swapped the 20" wheels for 17" SR5 wheels about three weeks later. The tire shop moved the TPMS sensors from the 20" Limited wheels to the 17" SR5 wheels, mounted new tires and installed on car. The spare was untouched. The TPMS light came on when I started the car, blinked for a few seconds, then lit steady - been doing this ever since...
The 2010 Limited wheels have the sensor attached to the tire stem, with the nut on the outside of the wheel. The spare tire is just a normal tire stem - and I assume there is no TPMS sensor inside - don't know why they would use a different type than the other four wheels on the car. Nothing has been done to the spare tire since new and there is no sensor in the glove box.
So I guess that does bring up a good question - is there a TPMS in the spare, and if so, why did they use a different type I wonder?