In theory you CAN wire the two batteries together, and in practice, if you never really discharge them much beyond using them to start the truck or just a few minutes of light use, you might not see any negative effects.
The problem is that the two batteries are dissimilar, in particular the charge/discharge capacity are different. Because of this, as they discharge, their internal resistance will differ from one another, and their corresponding voltage will differ. Since they are wired parallel, they will try to "equallize", meaning the one with the higher voltage will charge the one with lower voltage until their voltages are the same. This kind of cross current between the batteries can have a significant impact on the battery life for both batteries and will also affect their performance during duty.
Additionally, as the alternator starts to recharge the batteries, if they both do not require the same amount of charge to reach full charge at the same time, the battery that reaches full charge first will still accept a small amount of charge while the other charges. However, as it continues to overcharge, its resistance will exponentially increase, and generate a lot of heat. This of course can alter the long term performance of the battery and shorten its life.
If one of the batteries ever becomes damaged enough such that it is effectively shorted, it will draw all the charge it can from the alternator. Even though the other battery may be enough to sustain the vehicle, this becomes a safety issue as this this can generate a significant amount of heat in the damaged battery and can cause a fire.
You could run 2 batteries of the same make and model together (i.e. two Sears Die Hard type 24S batteries), but as they age and their internal chemistries and resistances change, you will run into the same problems.
The isolator works such that each battery will discharge separately (unless you have the type of isolator that allows you to manually switch both batteries together for emergencies only), and allows the alternator to charge each battery separately, so neither battery stands a chance of overcharging. The benefit of the isolator then, is safety, and battery life and performance. The type of isolator you choose depends on what type of duty you expect and the flexability and features you would like in your system.