Yes, all the turn signal lights should flash once for lock and twice for unlock.
For more information, review your Owner's Manual under Wireless Remote Control: Locking and Unlocking Doors.
This statement appears at the end of that section:
"The following adjustment can be made in this system. For details, contact your Toyota dealer - Cancelling the flash of the turn signal lights."
You may need to visit a Toyota dealer to have this feature turned back on.
Operation of Instruments and Controls - Keys and Doors
http://4runnerclub.com/i/4r_files/f...toyom7/toyompdf/07omsour/2007/074runne/12.pdf